Received: 2022-11-09 | Revised:2022-11-22 | Accepted: 2022-11-24 | Published: 2022-12-06
Pages: 75-87
DOI: https://doi.org/10.56717/jpp.2022.v01i02.010
Abstract
Yeasts infections, cancer and other diseases associated with free
radical generation and inflammation are currently a critical public health
issue that needs innovative control measures. In order to search for solutions,
this study was designed to assess the antifungal, radical scavenging,
anti-inflammatory, and antineoplastic activities of essential oils from four
Annonaceae plants (Monodora myristica, Xylopia aethiopica,
and Xylopia parviflora) collected in Chad and Cameroon. Essential
oils were extracted by hydrodistillation. The antifungal activity of the oils
was determined using the microdilution method; and their antiradical activity
was determined using the DPPH free radical scavenging assay. The
anti-inflammatory activity was assessed using the inhibitory effect of oils
against the 5-lipoxigenase. Flow cytometry was used to assess the effect of
essential oils on cell cycle. The essential oils of X. aethiopica, X.
parviflora, and M. myristica inhibited the growth of Candida
albicans ATCC24433, Candida parapsilosis ATCC22019,
and Cryptococcus neoformans IP95026 with MIC values ranging
from 5 to 10 mg/mL. Essential oils of X. aethiopica, X.
parviflora, and M. myristica showed free radical
scavenging potential with SC50 values between 8.9 and 11.74
g/L. The oils samples at 100 μg/mL from both origins significantly inhibited
cancer cell (MCF-7) and normal epithelial cells of the eye (ARPE-19) growth,
with a notable cell cycle arrest at phases G0/G1 and S at 72h respectively.
According to the findings of this study, essential oils from Annonaceae plants
contain bioactive secondary metabolites that have the potential to inhibit
pathogenic yeasts, free radicals, inflammatory and cancer cells. Further
research is needed to confirm these findings and formalize their eventual
application to control the targeted affections.
Abstract Keywords
Annonaceae, essential oil, chemical composition, anti-fungal, radical
scave-nging, anti-inflammatory, antineoplastic
References
1.
Karimi, E.; Jaafar, H. Z. E.; Ahmad, S.
Antifungal, anti-inflammatory and cytotoxicity activities of three varieties of
labisia pumila benth: from microwave obtained extracts. BMC Complement.
Alt. Med. 2013, 13,
20.
2.
Ferlay, J.; Colombet, M.; Soerjomataram, I.;
Parkin, D. M.; Piñeros, M.; Znaor, A.; Bray, F. Cancer statistics for the year
2020: An overview. Int. J. Cancer 2021.
3.
Deo, S.V.S.; Sharma, J.; and Kumar, S. GLOBOCAN
2020. Report on Global Cancer Burden: Challenges and Opportunities for
Surgical Oncologists. Ann. Surg Oncol, 2022. 29(11),
6497-6500.
4.
Allahqoli, L.; Mazidimoradi, A.;
Momenimovahed, Z.; Rahmani, A.; Hakimi, S.; Tiznobaik, A.; Gharacheh, M.;
Salehiniya, H.; Babaey, F.; Alkatout, I. The Global Incidence, Mortality, and
Burden of Breast Cancer in 2019: Correlation With Smoking, Drinking, and Drug
Use. Front.Oncol. 2022, 12.
5.
Ali, I.; Lone, M. N.; Aboul-Enein, H. Y.
Imidazoles as potential anticancer agents. Med. Chem. Comm. 2017, 8, 1742-1773.
6.
Dhiman, A.; Sharma, R.; Singh, R. K.
Target-based anticancer indole derivatives and insight into structure‒activity
relationship: A mechanistic review update (2018–2021). Acta Pharm.
Sinica B 2022, 12,
3006-3027.
7.
Sharifi-Rad, J.; Sureda, A.; Tenore, G. C.;
Daglia, M.; Sharifi-Rad, M.; Valussi, M.; Tundis, R.; Sharifi-Rad, M.; Loizzo,
M. R.; Ademiluyi, A. O.; Sharifi-Rad, R.; Ayatollahi, S. A.; Iriti, M.
Biological Activities of Essential Oils: From Plant Chemoecology to Traditional
Healing Systems. Molecules 2017, 22, 70.
8.
Granwehr, B. P.; Chemaly, R. F.; Kontoyiannis,
D. P.; Tarrand, J. J.: Chapter 44. Fungal and Viral Infections in Cancer
Patients. In The MD Anderson Manual of Medical Oncology, 2e;
Kantarjian, H. M., Wolff, R. A., Koller, C. A., Eds.; The McGraw-Hill
Companies: New York, NY, 2011.
9.
Alves, M.; Gonçalves, M. J.; Zuzarte, M.;
Alves-Silva, J. M.; Cavaleiro, C.; Cruz, M. T.; Salgueiro, L. Unveiling the
Antifungal Potential of Two Iberian Thyme Essential Oils: Effect on C. albicans
Germ Tube and Preformed Biofilms. Front. Pharmacol. 2019, 10.
10.
Choumessi, A. T.; Danel, M.; Chassaing, S.;
Truchet, I.; Penlap, V. B.; Pieme, A. C.; Asonganyi, T.; Ducommun, B.; Valette,
A. Characterization of the antiproliferative activity of Xylopia
aethiopica. Cell Div. 2012, 7, 8-8.
11.
Bakarnga-Via, I.; Hzounda, J. B.; Fokou, P. V.
T.; Tchokouaha, L. R. Y.; Gary-Bobo, M.; Gallud, A.; Garcia, M.; Walbadet, L.;
Secka, Y.; Dongmo, P. M. J.; Boyom, F. F.; Menut, C. Composition and cytotoxic
activity of essential oils from Xylopia aethiopica (Dunal) A.
Rich, Xylopia parviflora (A. Rich) Benth.) and Monodora
myristica (Gaertn) growing in Chad and Cameroon. BMC
Complement. Alter. Med. 2014, 14,
125.
12.
Ngane, A. N.; Biyiti, L.; Zollo, P. H. A.;
Bouchet, P. Evaluation of antifungal activity of extracts of two Cameroonian
Rutaceae: Zanthoxylum leprieurii Guill. et Perr. and Zanthoxylum
xanthoxyloides Waterm. J. Ethnopharmacol. 2000, 70, 335-342.
13.
Brand-Williams, W.; Cuvelier, M. E.; Berset,
C. Use of a free radical method to evaluate antioxidant activity. LWT -
Food Sci. Tech. 1995, 28,
25-30.
14.
Dongmo, P. M. J.; Tchoumbougnang, F.;
Ndongson, B.; Agwanande, W.; Sandjon, B.; Zollo, P. H. A.; Menut, C. Chemical
characterization, antiradical, antioxidant and anti-inflammatory potential of
the essential oils of Canarium schweinfurthii and Aucoumea
klaineana (Burseraceae) growing in Cameroon. Agric. Biol. J.
North America 2010, 1,
606-611.
15.
Braga, M. E. M.; Ehlert, P. A. D.; Ming, L.
C.; Meireles, M. A. A. Supercritical fluid extraction from Lippia alba: global
yields, kinetic data, and extract chemical composition. J. Supercrit.
Fluids 2005, 34,
149-156.
16.
Gary-Bobo, M.; Brevet, D.; Benkirane-Jessel,
N.; Raehm, L.; Maillard, P.; Garcia, M.; Durand, J. O. Hyaluronic
acid-functionalized mesoporous silica nanoparticles for efficient photodynamic
therapy of cancer cells. Photodiagnosis Photodyn. Ther. 2012, 9, 256-260.
17.
Giret, S.; Théron, C.; Gallud, A.; Maynadier,
M.; Gary-Bobo, M.; Garcia, M.; Wong Chi Man, M.; Carcel, C. A designed
5-fluorouracil-based bridged silsesquioxane as an autonomous acid-triggered
drug-delivery system. Chemistry 2013, 19, 12806-12814.
18.
Chalchat, J.-C.; Garry, R.-P.; Menut, C.;
Lamaty, G.; Malhuret, R.; Chopineau, J. Correlation Between Chemical
Composition and Antimicrobial Activity. VI. Activity of Some African Essential
Oils. J. Essent. Oil Res. 1997, 9, 67-75.
19.
Tatsadjieu, L. N.; Essia Ngang, J. J.;
Ngassoum, M. B.; Etoa, F. X. Antibacterial and antifungal activity of Xylopia
aethiopica, Monodora myristica, Zanthoxylum xanthoxyloi;des
and Zanthoxylum leprieurii from Cameroon. Fitoterapia 2003, 74, 469-472.
20.
Filipowicz, N.; Kamiński, M.; Kurlenda, J.;
Asztemborska, M.; Ochocka, J. R. Antibacterial and antifungal activity of
juniper berry oil and its selected components. Phytother. Res. 2003, 17, 227-231.
21.
Dongmo, P. M. J.; Tatsadjieu, L. N.;
Tchoumbougnang, F.; Sameza, M. L.; Dongmo, B. N.; Zollo, P. H. A.; Menut, C.
Chemical Composition, antiradical and antifungal activities of essential oil of
the leaves of Cinnamomum zeylanicum blume from Cameroon. Nat.
Prod. Com. 2007, 2.
22.
Aouni, M.; Pelen, F.; Soulimani, R. Étude de l’activité
antimicrobienne d’un mélange de 41 huiles essentielles et domaines
d’application. Phytothér. 2013, 11,
225-236.
23.
Jean Baptiste, H.; Jazet Dongmo, P. M.; V.I,
B.; Ngo Mback, M. N. L.; M.E, Z. O.; Fall, A.; Bassene, E. Optimized
Combinations of Ocimum Essential Oils Inhibit Growth of Four Candida
albicans. Int. J. Drug Dis. 2014, 6, 198-206.
24.
Peana, A. T.; D'Aquila, P. S.; Panin, F.;
Serra, G.; Pippia, P.; Moretti, M. D. L. Anti-inflammatory activity of linalool
and linalyl acetate constituents of essential oils. Phytomed. 2002, 9, 721-726.
25.
Pitarokili, D.; Tzakou, O.; Loukis, A.;
Harvala, C. Volatile Metabolites from Salvia fruticosa as Antifungal Agents in
Soilborne Pathogens. J. Agric. Food Chem. 2003, 51, 3294-3301.
26.
Carbonnelle, B. F. M., D. A.; Pinon, G.; Vargues,
R.: Bactériologie Medicale. Techniques
Usuelles. 330 pages. ISBN 2 85334 276 X. SIMEP, Paris, 1987, FF 480; 2009/04/06 ed.; Cambridge University Press, 1987; Vol. 96.
27.
Alitonou, G.; Tchobo, F.; Avlessi, F.;
Sohounhloue, D. K.; Menut, C. Aeollanthus pubescens Benth. from Benin: A
Potential Source of Essential Oil with High Antiradical Efficiency. J.
Essent. Oil Bear. Pl. 2013, 16,
308-314.
28.
Woguem, V.; Fogang, H. P.; Maggi, F.;
Tapondjou, L. A.; Womeni, H. M.; Quassinti, L.; Bramucci, M.; Vitali, L. A.;
Petrelli, D.; Lupidi, G.; Papa, F.; Vittori, S.; Barboni, L. Volatile oil from
striped African pepper (Xylopia parviflora, Annonaceae) possesses
notable chemopreventive, anti-inflammatory and antimicrobial potential. Food
Chem. 2014, 149,
183-189.
29.
Leers, M. P.; Kölgen, W.; Björklund, V.;
Bergman, T.; Tribbick, G.; Persson, B.; Björklund, P.; Ramaekers, F. C.;
Björklund, B.; Nap, M.; Jörnvall, H.; Schutte, B. Immunocytochemical detection
and mapping of a cytokeratin 18 neo-epitope exposed during early
apoptosis. J. Pathol. 1999, 187,
567-572.
30.
Szmit, E.; Trzonkowski, P.; Myśliwska, J.;
Foerster, J.; Myśliwski, A. Ex vivo apoptotic potential of
peripheral blood mononuclear cells of the elderly human subject. Cell
Biol. Inter. 2002, 26,
517-527.
31.
Patel, P.B.; Thakkar, V.R.; Patel,
J.S. Cellular Effect of Curcumin and Citral Combination on Breast Cancer
Cells: Induction of Apoptosis and Cell Cycle Arrest. J. Breast. Cancer., 2015. 18(3), 225-234.
32.
Sarasin, A. Les gènes humains de la réparation de
l'ADN. Med. Sci. 2013, 10, 43.
33.
Sarasin, A. La réparation de l'ADN au centre de la
biologie de la cellule. Med.
Sci. (Paris) 1994, 10,
951-952.
34.
Cha, J. D.; Kim, J. Y. Essential oil from
Cryptomeria japonica induces apoptosis in human oral epidermoid carcinoma cells
via mitochondrial stress and activation of caspases. Molecules 2012, 17, 3890-3901.
35.
Wei, F. X.; Li, M. Y.; Song, Y. H.; Li, H. Z.
[Apoptosis and activity changes of telomerase induced by essential oil from
pine needles in HepG2 cell line]. Zhong Yao Cai 2008, 31, 1197-1200.
This work is licensed under the
Creative Commons Attribution
4.0
License (CC BY-NC 4.0).
Abstract
Yeasts infections, cancer and other diseases associated with free
radical generation and inflammation are currently a critical public health
issue that needs innovative control measures. In order to search for solutions,
this study was designed to assess the antifungal, radical scavenging,
anti-inflammatory, and antineoplastic activities of essential oils from four
Annonaceae plants (Monodora myristica, Xylopia aethiopica,
and Xylopia parviflora) collected in Chad and Cameroon. Essential
oils were extracted by hydrodistillation. The antifungal activity of the oils
was determined using the microdilution method; and their antiradical activity
was determined using the DPPH free radical scavenging assay. The
anti-inflammatory activity was assessed using the inhibitory effect of oils
against the 5-lipoxigenase. Flow cytometry was used to assess the effect of
essential oils on cell cycle. The essential oils of X. aethiopica, X.
parviflora, and M. myristica inhibited the growth of Candida
albicans ATCC24433, Candida parapsilosis ATCC22019,
and Cryptococcus neoformans IP95026 with MIC values ranging
from 5 to 10 mg/mL. Essential oils of X. aethiopica, X.
parviflora, and M. myristica showed free radical
scavenging potential with SC50 values between 8.9 and 11.74
g/L. The oils samples at 100 μg/mL from both origins significantly inhibited
cancer cell (MCF-7) and normal epithelial cells of the eye (ARPE-19) growth,
with a notable cell cycle arrest at phases G0/G1 and S at 72h respectively.
According to the findings of this study, essential oils from Annonaceae plants
contain bioactive secondary metabolites that have the potential to inhibit
pathogenic yeasts, free radicals, inflammatory and cancer cells. Further
research is needed to confirm these findings and formalize their eventual
application to control the targeted affections.
Abstract Keywords
Annonaceae, essential oil, chemical composition, anti-fungal, radical
scave-nging, anti-inflammatory, antineoplastic
This work is licensed under the
Creative Commons Attribution
4.0
License (CC BY-NC 4.0).
Editor-in-Chief
This work is licensed under the
Creative Commons Attribution 4.0
License.(CC BY-NC 4.0).