Research Article
Received: 2022-11-11 | Revised:2022-11-22 | Accepted: 2022-12-05 | Published: 2022-12-05
Pages: 66-74
DOI: https://doi.org/10.56717/jpp.2022.v01i02.009
Abstract
Worldwide
attention on plant study has expanded, and a wealth of data has accumulated to
demonstrate the enormous potential of medicinal plants employed in diverse
traditional systems. In the traditional medical system, Flacourtia
indica (FI) has been used to cure a variety of illnesses, including
cancer, diabetes, hepatic disorders, and snakebites. Due to the presence of a
variety of phytochemicals, including alkaloids, flavonoids, tannins,
terpenoids, glycosides, it may have antioxidant and anti-inflammatory activity.
Thus, this study was designed to assess the analgesic, anti-inflammatory and
antipyretic effects of the ethanolic extract of Flacourtia indica. Flacourtia indica was extracted using 100% ethanol followed
by assessing acute toxicity and doses selected for
the studies were 500 and 1000 mg/kg body weight. Swiss albino mice
of either sex weighing 25-30 gm and Sprague dawley rats
weighing 180-200 gm were used in this study. Analgesic activity was
evaluated by using acetic acid induced writhing test, formalin induced paw
licking and hot plate test. Anti-inflammatory effect was assessed using xylene
and croton oil induced ear edema test and carrageenan induced paw edema test.
Also the antipyretic effect was investigated. The Flacourtia
indica extract exhibited significant effect against pain in acetic
acid test (p<0.01), formalin test (p<0.01) and insignificantly in hot
plate test. Inflammation was reduced by FI extract in xylene test (p<0.05),
croton oil test (p<0.01) and significantly reduced the paw edema
(p<0.001) in carrageenan-induced paw edema test. In the yeast-induced
antipyretic test, administration of Flacourtia indica at both
doses significantly (p< 0.001) reduced pyrexia. Within
these two doses, higher doses (1000 mg/kg body weight) had better aptitudes in
the reduction of pain, inflammation and pyrexia. The FI extract shown
strong analgesic, anti-inflammatory, and antipyretic capabilities, according to
the study results, and more research is needed to assess these effects and the
potential of the plants.
Abstract Keywords
Medicinal
plant, Flacourtia Indica, analgesic effect, anti-inflammatory
effect, yeast induced pyrexia.
References
1. Thirunethiran,
K.; Varalakshmi, B.; Suguna Bai, J.; Gomathi, S. Effect of different doses
of cucurbita pepo linn extract as an anti-inflammatory and analgesic
nutraceautical agent on inflamed rats. Int. J. Phar. Res. Dev, 2011, 3(7), 60-78.
2.
Estakhr, J.; Sanchooli, N.; Najafi, S.H.; Javdan, N. Anti-Inflammatory
Activity of Ethanolic Extract of Physalis alkekengi. Res.
J. Pharm. Biol. Chem. Sci. 2011,
2(3), 421-425.
3.
Wohlmuth, H.; Oliver, C.; Nathan, P.J. A review of the status of Western
herbal medicine in Australia. J. Herb. Pharmacother, 2002, 2, 33-46.
4. http://www.floraofbangladesh.com/2020/05/boichi-or-madagascar-plum-flacourtia.html.
5.
Eramma, N.K. A Comprehensive Review on Pharmacology of Flacourtia
Indica (BURM.F.) Merr. (governor’s Plum). Int. J. Pharm. Chem.
Sci. 2016, 5(3).
6.
Kirtikar, K.R.; Basu, B.D. Indian Medicinal Plants. 3rd ed. Vol II, Singh
and MP Singh Publications. India. 1998,
220.
7. Kaou,
A.M.; Leddet, V.M.; Canlet. C.; Debrauwer, L.; Hutterm, S. Antimalarial
compounds from aerial parts of Flacourtia indica (Flacoutiaceae). J
Ethnopharmacol. 2010,
130(2), 272-274.
8.
Pachute, P.A.; Tyagi, S.; Mishra, A.; Shakya, A.; Kumar, D.; Patel, V.K.
Preliminary evaluation of Anticancer activity of Flacourtia indica Merr. Bot.
Res. Int. 2011,
4(3),43-47
9. Asad,
B.H.H.M.; Murtaza, G.; Siraj, S.; Khan, A.S.; Azhar, S.; Hussain, S.M.; Ismail,
T.; Hussain, S.M.; Hussain, I. Enlisting the scientifically unnoticed medicinal
plants of Pakistan as a source of novel therapeutic agents showing anti-venom
activity. Afr. J. Pharma. Pharmacol. 2011, 5(20), 2292-2305.
10. Kim,
J.S.; Ju, J.B.; Choi, C.W.; Kim, S.C.; Hypoglycemic and antihyperlipidemic
effect of four Korean Medicinal plants in alloxan induced diabetes rats. Amer.
J. Biochem. Biotech. 2006,
2(4), 154-160.
11. Koster,
R.; Anderson, M.; De-Beer, E.J. Acetic acid analgesic screening. Fed.
Proc. 1959, 18,
412–417.
12. Eddy,
N.B.; Leimbach, D. Synthetic Analgesics II. Dithienylbutenyl- And
Dithienylbutylamines. J. Pharmacol. Exp. Therap. 1953, 107(3), 385-93.
13. Hunskaar,
S.; Hole, K. The formalin test in mice: dissociation between inflammatory and
non-inflammatory pain. Pain 1987, 30, 103-114.
14. Vinegar,
R.; Schreiber, W.; Hugo R, Biphasic development of carrageenan-induced oedema
in rats. J. Pharmacol. Exp. Ther. 1969, 166, 96-103.
15. Dai,
Y.; Liu, L.H.; Kou, J.P.; Anti-inflammatory effect of aqueous extract of
Wu-HU-Tang. China. Pharm. Uni. 1995, 6, 362-364.
16. Swingle,
K.F.; Shideman, F.E. Phases of the inflammatory response to subcutaneous
implantation of a cotton pellet and their modification by certain
anti-inflammatory agents. J. Pharmacol. Exp. Ther. 1972, 185, 226–234.
17. Loux,
J.J.; De Palma, P.D.; Yankell, S.L. Antipyretic testing of aspirin in
rats. Toxicol. Appl. Pharmacol. 1972, 22, 672–675.
18. Victor,
B.O.; Caleb, O.W.; Ayodele, O. S.; Samuel, B.O. J. Ethnopharmacol. 2004, 90, 317-321.
19. Vyklicky,
L.Techniques for the study of pain in animals, In: Bonica, J.J.;
Liebeskin & Albe-Fessard, D.G. (Eds), Advances in Pain Research and
Therapy. Raven. New York, USA, 1979,
773-778.
20. Vongtau,
H.O.; Abbah, J.; Mosugu, O., et al. Antinociceptive profile of the ethanolic
extract of Neorautanenia mitis root in rats and mice. J.
ethnopharmacol. 2004,
92(2-3), 317- 324.
21. Tjolsen,
A.; Gerge, O.G.; Hunskaar, S., et al. The formalin test: an evaluation of
method. Pain 1992, 51,
3-17.
22. Ghannadi,
A.; Hajhashemi, V.; Jafarabadi, H. An investigation of the analgesic and
anti-inflammatory effects of Nigella sativa seed
polyphenols. J. Medicinal. Food. 2005, 8, 488-493.
23. Gabor,
M. Mouse Ear Inflammation Models and
their Pharmacological Applications. 2000, Akadémiai Kiadó, Budapest, Hungary.
24. Halliwell,
B.; Gutteridge, J.M.C. Free radicals in biology and medicine, 1999, Oxford University press.
25. Conner,
E.M.; Grisham, M.B. Inflammation, free radicals, and
antioxidants. Nutrition 1996,
12(4), 274-277.
26. Collier,
H.O.; Dinneen, L.C.; Johnson, C.A.; Schneider, C. The
abdominal constriction response and its suppression by analgesic drugs in the
mouse. Br. J. Pharmacol. 1968, 32(2), 295–310.
27. Deraedt,
R.; Jouquey, S.; Delevallée, F.; Flahaut, M. Release of
prostaglandins E and F in an algogenic reaction and its inhibition. Eur.
J. Pharmacol. 1980,
61(1), 17-24.
28. Roberts,
L.J.; Morrow, J.D. Analgesic, antipyretic and Anti-inflammatory Agents and
Drugs Employed in the Treatment of Gout. In: Eds. Goodman and Gilman’s The
Pharmacological Basis of Therapeutics. 10th ed., McGraw-Hill. New York; 2001, 687-732.
29. Richardson, Kilob,
S.; Hargreavesab,
K.M. Cannabinoids reduce hyperalgesia and inflammation via interaction
with peripheral CB1 receptors. Pain 1998, 75(1),111-119.
30. Bensreti,
M.M.; Sewell, R.D.E. Selective effects of dopaminergic modifiers on
antinociception produced by different opioid receptoragonists. Pro. Br.
Pharmacol. Soc. 1983,
6th – 8th July,70.
31. Headley,
P.M.; O’Shaughnessy, C.T. Evidence for opiate and dopamine interaction in
striatum. Br. J. Pharmacol. 1985, 86 (pro. Suppl.), 700.
32. Wigdor,
S.; Wilcox, G.L. Central and systemic morphine-induced antinociception in mice:
Contribution of descending serotonergic and noradrenergic pathways. J.
Pharmacol. Exp. Ther. 1987,
242, 90-95.
33. Pasero,
C.; Paice, J.A.; McCaffery, M. Basic Mechanisms underlying the causes and
effects of pain. In Pain, McCaffery M, Pasero C (eds). Mosby: St. Louis 1999, 15- 34.
34. Salvemini, D.; Wang, Z.-Q.; David, M.B.; Michael, K.S.; Mark, G.C.; Pamela, T.M. Evidence of peroxynitrite involvement in
the carrageenan-induced rat paw edema. Eur. J. Pharmacol. 1996, 303(3), 217-220.
35. Loram, L.C.; Fuller, A.; Fick, L.G.; Cartmell,
T.; Poole, S.; Mitchell, D. Cytokine Profiles During
Carrageenan-Induced Inflammatory Hyperalgesia in Rat Muscle and Hind Paw. J.
Pain 2007, 8, 127-136.
36. Ribeiro,
R.V.; Matos da Silva, R.; Joaquim Lima, J.C.S.; Martins, D.T.O.
Antiinflammatory, antinociceptive and antipyretic effects of hydroethanolic
extract from Macrosiphonia velame (A. St.-Hil.) M. Arg. in animal models. Braz.
J. Pharm. Sci. 2010,
46(3), 515-523.
37. DiMartino,
M.J.; Campbell, J.R.G.K.; Wolff, C.E.; Hanna, N.; The pharmacology of
arachidonic acid-induced rat paw edema. Agents and Actions 1987, 21 (3-4), 303-305.
38. Luber-Narod,
J.; Austin-Ritchie, T.; Hollins, C.; Menon, M.; Malhotra, R.K.; Baker, S.; et
al. Urol. Res. 1997,
25, 395–9.
39. Atta,
A.H.; Alkofahi, A. Anti-nociceptive and anti-inflammatory effects of some
Jordanian medical plant extracts. J. Ethnopharm. 1998, 60,117-124.
40. Kavimani,
S.; Vetrichelvan, T.; Ilango, R.; Jaykar, B. Antiinflammatory activity of the
volatile oil of Toddalia asiatica. Indian.
J. Pharm. Sci. 1996,
58(2), 67–70.
41. Simmons,
D.L.; Botting, R.M.; Hla, T. Cyclooxygenase isozymes: the biology of
prostaglandin synthesis and inhibition. Pharmacol. Rev. 2004, 56, 387-437.
This work is licensed under the
Creative Commons Attribution
4.0
License (CC BY-NC 4.0).
Abstract
Worldwide
attention on plant study has expanded, and a wealth of data has accumulated to
demonstrate the enormous potential of medicinal plants employed in diverse
traditional systems. In the traditional medical system, Flacourtia
indica (FI) has been used to cure a variety of illnesses, including
cancer, diabetes, hepatic disorders, and snakebites. Due to the presence of a
variety of phytochemicals, including alkaloids, flavonoids, tannins,
terpenoids, glycosides, it may have antioxidant and anti-inflammatory activity.
Thus, this study was designed to assess the analgesic, anti-inflammatory and
antipyretic effects of the ethanolic extract of Flacourtia indica. Flacourtia indica was extracted using 100% ethanol followed
by assessing acute toxicity and doses selected for
the studies were 500 and 1000 mg/kg body weight. Swiss albino mice
of either sex weighing 25-30 gm and Sprague dawley rats
weighing 180-200 gm were used in this study. Analgesic activity was
evaluated by using acetic acid induced writhing test, formalin induced paw
licking and hot plate test. Anti-inflammatory effect was assessed using xylene
and croton oil induced ear edema test and carrageenan induced paw edema test.
Also the antipyretic effect was investigated. The Flacourtia
indica extract exhibited significant effect against pain in acetic
acid test (p<0.01), formalin test (p<0.01) and insignificantly in hot
plate test. Inflammation was reduced by FI extract in xylene test (p<0.05),
croton oil test (p<0.01) and significantly reduced the paw edema
(p<0.001) in carrageenan-induced paw edema test. In the yeast-induced
antipyretic test, administration of Flacourtia indica at both
doses significantly (p< 0.001) reduced pyrexia. Within
these two doses, higher doses (1000 mg/kg body weight) had better aptitudes in
the reduction of pain, inflammation and pyrexia. The FI extract shown
strong analgesic, anti-inflammatory, and antipyretic capabilities, according to
the study results, and more research is needed to assess these effects and the
potential of the plants.
Abstract Keywords
Medicinal
plant, Flacourtia Indica, analgesic effect, anti-inflammatory
effect, yeast induced pyrexia.
This work is licensed under the
Creative Commons Attribution
4.0
License (CC BY-NC 4.0).
Editor-in-Chief
This work is licensed under the
Creative Commons Attribution 4.0
License.(CC BY-NC 4.0).